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3/7 ME-180 SiHa, caspase and Cannabis Effect activity and of HeLa, cells cannabidiol sativa of on



  • 3/7 ME-180 SiHa, caspase and Cannabis Effect activity and of HeLa, cells cannabidiol sativa of on
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  • effects of crude extract of Cannabis sativa and its main compound cannabidiol 6 Caspase 3/7 activity after treatment of SiHa, HeLa, and ME cells with IC. Effect of Cannabis sativa extracts and cannabidiol on cell growth of cervical . Caspase 3/7 activity after treatment of SiHa, HeLa, and ME The aim of this study was to evaluate for the anti-growth effects of Cannabis sativa extracts and its isolate, cannabidiol on cervical cancer cell lines HeLa, SiHa, and ME Cannabis sativa extracts led to the up-regulation of apoptosis an increase in Caspase 3/7 and a decrease in the ATP levels.

    3/7 ME-180 SiHa, caspase and Cannabis Effect activity and of HeLa, cells cannabidiol sativa of on

    Statistical analysis of the graphical data was expressed as the mean standard deviation. Camptothecin, as our positive control, significantly reduced cell viability in SiHa As shown in Fig. Whereas ethanol exhibited between 7. Representative cell viability bar graphs of cervical cancer cell lines. The IC 50 obtained during MTT assay was tested for their ability to alter cell viability in real time.

    Cells were seeded in an E-plate and allowed to attach. Continuous changes in the impedance were measured and displayed as cell index CI. Little can be read from xCELLigence except that cannabidiol in all cell lines has shown to reduce cell index while the plant extract had mixed results sometimes showing reduction on the other hand remained unchanged Fig. Suggesting that cannabidiol is the most effective compound.

    BXfluorescence confocal microscopy was used to visualize the cells. Apoptosis assessment following treatment of cervical cancer cells with IC 50 concentrations of Cannabis sativa extract and cannabidiol. These bar graphs are a representative of apoptosis induction in SiHa a and d , HeLa b and e , and ME c and f cells. Flow cytometry revealed a significant increase in SiHa cells undergoing apoptosis during treatment with butanol from 2 to In HeLa cells, apoptosis was increased to A similar events was observed following treatment of ME cells with butanol extract were Cannabidiol was also tested for its ability to induce apoptosis in all three cell lines.

    The results further confirmed that the type of cell death induced was apoptosis. Figure shows that cannabidiol induced early apoptosis in all three cell lines. Cannabidiol was more effective in inducing apoptosis In comparison to both extracts of Cannabis sativa.

    In SiHa cells cannabidiol induced To characterise the cell death type following treatment with our test compounds, cell were stained with DAPI and Annixin V to show if apoptosis was taking place. Treatment of SiHa and HeLa cells with IC 50 of both butanol and hexane extracts confirmed the type of cell death as apoptosis since they picked a green colour from Annexin V that bind on phosphotidyl molecules that appear in early stages of apoptosis.

    Similar results were also observed in cannabidiol treated cells. Another feature that is a representative of cell death is the change in morphology.

    Morphological appearance of live cells displayed a round blue nuclei following staining with DAPI. Loss of shape, nuclear fragmentation, reduction in cell size and blebbing of the cell membrane were among the observed morphological features implicated to be associated with apoptosis Fig.

    Bar graphs representing changes in the ATP levels following treatment of cervical cancer cells with Cannabis sativa and cannabidiol. Untreated and camptothecin were included as controls for comparative purposes.

    This was done in order to determine whether Cannabis sativa and cannabidiol deplete ATP levels in cervical cancer cells. In general, ATP depletion was cell type dependent. Whereas in ME there was no change between treatments and untreated. Similar results were observed in cannabidiol treated cells.

    This could mean that cannabidiol depletes ATP levels more than Cannabis sativa extracts and might be the main compound responsible for cell death in cancer cells treated with Cannabis sativa. There was no significant change in ME cells Figure. ME was fairly increased also to from RLU all increase were significant and in line with other increase in apoptosis as shown in Annexin V. Representative bar graph of the cervical cancer cell cycle before and after treatment with Cannabis sativa extracts and cannabidiol.

    Cells were harvested and treated with camptothecin and the IC 50 concentrations of Cannabis sativa extracts and cannabidiol. We further assessed the effects of Cannabis sativa extracts and cannabidiol on cell cycle progression using flow cytometry. In butanol, sub-G 0 phase was increased from 4.

    In ME there was insignificant increase in all cell cycle stages. Each cell line responded differently to cannabidiol treatment. A similar trend was observed in HeLa cells but much lower sub-G 0 from 5. A similar event was observed during treatment of ME cells. Cannabidiol significantly increased sub-G0 in ME cells to From this data, we can conclude that cannabidiol induced cell death without cell cycle arrest. A densitometry analysis SiHa protein was performed using ImageJ quantification software to measure the relative band intensity.

    The genes analyzed are p53 and RBBP6 including caspases. Equal amount of protein conc was loaded in each well. Note that the darker the bands increased expression of the gene. A densitometry analysis HeLa protein was performed using ImageJ quantification software to measure the relative band intensity.

    A densitometry analysis ME protein was performed using ImageJ quantification software to measure the relative band intensity. Untreated protein was used as a control. Antibodies against pro-apoptotic proteins p53 and Bax and anti-apoptotic proteins Bcl-2 and RBBP6 , Initiator caspase-9 and effecter caspase-3 were included to elucidate apoptosis induction. From the apoptosis experiments conducted, it is clear that the mode of cell death induced by cannabidiol and extract of Cannabis Savita was that of apoptosis.

    However, we needed to confirm whether the type of apoptosis induced is it p53 dependent or independent as it is well known that p53 is mutated in many cancers. Similar results were observed in hexane treated cells. In all cell lines the level of p53 negative regulator in cancer development was reduced by all treatment. Following treatment of cervical cancer cells, Bax protein was up-modulated and Bcl-2 was down-modulated.

    Western blot analysis revealed that cannabidiol effectively caused an increase in the expression of pro-apoptosis proteins, p53 and Bax, while simultaneously decreasing the anti-apoptosis proteins, RBBP6 and Bcl-2 in all three cervical cancer cell lines SiHa, HeLa, and ME cells.

    Caspases play an effective role in the execution of apoptosis, an effector caspase-9 and executor capsase-3 were included in our western blot to check if they played a role in inducing apoptosis. In all Cannabis sativa extracts, caspase-3 and caspase-9 were upregulated in all cell lines. Similar results were also observed in cannabidiol treated cells with upregulation of both caspase-3 and Cervical cancer remains a burden for women of Sub-Saharan Africa.

    Half a million new cases of cervical cancer and a quarter of a million deaths are reported annually due to lack of effective treatment [ 12 ]. Currently, the recommended therapeutic regimens include chemotherapy, radiation therapy, and surgery.

    However, they present several limitations including side effects or ineffectiveness [ 2 ]. Therefore, it is important to search for new novel therapeutic agents that are naturally synthesized and cheaper, but still remain effective. Medicinal plants have been used for decades for health benefits and to treat several different diseases [ 22 ]. However, some of the medicinal plants used by these individuals are not known to be effective and their safety is still unclear.

    It is therefore important to scientifically evaluate and validate their efficacy and safety. In the present study, cervical cancer cell lines SiHa, HeLa, and ME were exposed to different concentrations of Cannabis sativa extracts and that of its compound, cannabidiol, with the aim of investigating their anti-proliferative activity.

    We first determined whether Cannabis sativa extracts and cannabidiol possess anti-proliferative effects using MTT assay. These results correlate with the findings obtained by [ 23 ], whereby they reported reduced cell proliferation in colorectal cancer cell lines following treatment with Cannabis sativa.

    It was suggested that cannabidiol might be responsible for the reported activities. Therefore, in this study, cannabidiol was included as a reference standard in order to determine whether the reported pharmacological activities displayed by Cannabis sativa extracts might have been due to the presence of this compound. For positive extract inhibitory activity, Camptothecin was included as a positive control.

    Camptothecin functions as an inhibitor of a topoisomerase I enzyme that regulates winding of DNA strands [ 19 , 20 ]. This in turn causes DNA strands to break in the S-phase of the cell cycle [ 20 ]. A study conducted by [ 19 ], exhibited the ability of camptothecin to be cytotoxic against MCF-7 breast cancer cell line and also induce apoptosis as a mode of cell death at 0.

    We also observed a similar cytotoxic pattern, whereby camptothecin induced cell death in HeLa, SiHa, and ME cells, however, at a much higher concentration. Upon treatment of SiHa and HeLa cells with IC 50 of butanol extract, we noted that there was little to no inhibitory effect observed on cell growth.

    The growth curve continued in its exponential growth in all cells including the treated, untreated and 0. Differences in the findings could be attributable to the fact that both methods have different principles and mechanism of action.

    MTT assay is an end-point method that is based on the reduction of tetrazolium salt into formazan crystals by mitochondrial succinate dehydrogenase enzyme. Mitochondrial succinate dehydrogenase is only active in live cells with an intact metabolism [ 8 , 13 ]. Induction of cell death by Cannabis sativa crude extracts decreases the activity of the enzyme following treatment of HeLa, SiHa, and ME cervical cancer cell lines.

    On the other hand, xCELLigence system is a continuous method that relies on the use of E-plates engraved with gold microelectrodes at the bottom of the plate.

    The xCELLigence system is based on the changes in impedance influenced by cell number, size and attachment [ 13 ]. Therefore, we concluded that it was possible that dead cells might have been attached at the bottom of the E-plate after treatment. Cell death can be characterized by a decrease in the energy levels as a result of dysfunction of the mitochondria [ 8 ].

    Therefore, to evaluate the effect of treatment on the energy content of the cells, we conducted mitochondrial assay. ATP acts as determinant of both cell death and cell proliferation [ 15 ].

    Treatment of cells with cannabidiol either slightly or severely depleted the ATP levels. According to [ 16 ], a reduction of the ATP levels compromises the status of cell and often leads to cell death either by apoptosis or necrosis, while an increase is indicative of cell proliferation. Therefore, we concluded that the reduction of ATP might have been as a result of cell death induction since the cells ATP production recovered.

    Following confirmation that Cannabis sativa and cannabidiol have anti-proliferative activity, we had to verify whether both treatments have the ability to induce cell cycle arrest in all three cell lines. This method uses a PI stain and flow cytometry to measure the relative amount of DNA present in the cells. In this study, propidium iodide PI was used to stain cells. Propidium iodide can only intercalate into the DNA of fixed and permeabilized cells with a compromised plasma membrane or cells in the late stage of apoptosis.

    Viable cells with an intact plasma membrane cannot uptake the dye. The intensity of stained cells correlates with the amount of DNA within the cells. Treatment of SiHa cells with butanol and hexane extracts led to the accumulation of cells in the cell death phase sub-G 0 phase , without cell cycle arrest.

    And thus, according to [ 3 ], signals DNA synthesis and cell cycle proliferation. Interesting to note was that, treatment of ME cells with both extracts led to an increase of cells coupled by an increase in the S-phase population which favours replication and duplication of DNA. This was not the case following treatment of cells with cannabidiol. Cannabidiol resulted in the accumulation of cells in the cell death phase of the cell cycle.

    In summary, Cannabis sativa induces cell death with or without cell cycle arrest while cannabidiol induces cell death without cell cycle arrest. Apoptosis plays a major role in determining cell survival. Viable cells cannot uptake both dyes due to the presence of an intact cell membrane. Since treatment caused the accumulation of cells in the sub-G 0 phase, also known as the cell death phase, and the severe depletion of ATP levels by cannabidiol, we further conducted an apoptosis assay.

    Treatment of all three cell lines with camptothecin, IC 50 of Cannabis sativa and cannabidiol exhibited the type of induced cell death as apoptosis. Apoptosis is characterized by morphological changes and biochemical features which include condensation of chromatin, convolution of nuclear and cellular outlines, nuclear fragmentation, formation of apoptotic blebs within the plasma membrane, cell shrinkage due to the leakage of organelles in the cytoplasm as well as the presence of green stained cells at either late or early apoptosis [ 5 , 17 , 28 ].

    This also proves that during cell growth analysis, SiHa and HeLa cells were undergoing cell death while still attached to the surface of the flask. Apoptosis is known to occur via two pathways, the death receptor pathway and the mitochondrial pathway [ 30 ]. Cannabis sativa isolates including cannabidiol have been implicated in apoptosis induction via the death receptor pathway, by binding to Fas receptor or through an activated of Bax triggered by the synthesis of ceramide in the cells [ 4 ].

    However, not much has been reported on the induction of apoptosis via activation of p53 by Cannabis sativa. Our focus in this study was also to identify downstream molecular effect of extracts. The chloroform fraction of ES is the most cytotoxic fraction against T47D cells without prolonging the cell lifecycle. Anticancer potential of Hericium erinaceus extracts against particular human cancer cell lines.

    Full Text Available Cancer is a leading cause of death worldwide. Cancer resulted in 8. It is expected that annual cancer cases will rise from 14 million in to 22 million within the next two decades. Mushrooms are extensively used as nutritional supplements in many countries. Moreover, mushrooms have many medicinal properties, including anticancer activity. In this study, the anticancer activity of different polar and non-polar extracts of Hericium erinaceus were evaluated against different human cancer cell lines including human liver carcinoma Hep G2, the human colonic epithelial carcinoma HCT , the human cervical cancer cells HeLa and the human breast adenocarcinoma MCF-7 using 3- 4,5-Dimethylthiazolyl-2,5-diphenyltetrazolium bromide MTT assay.

    Furthermore, as a control, the cytotoxicity effect of the different extracts were tested against isolated mouse hepatocytes. It was observed that the extracts by water and methanol from fresh and lyophilized fruiting bodies of H. In contrast, the extracts by ether and ethyl acetate from mycelia and broth of H. The highest anticancer activity was recorded for aqueous extract of lyophilized fruiting bodies with half maximal inhibitory concentration IC50 values of 6.

    To summarise, polar extracts of H. I recommend further chemical studies to isolate the active principles of the extract of H. Cytotoxic activity of methanol extracts from Basidiomycete mushrooms on murine cancer cell lines.

    Crude methanol extracts of 58 mushroom species were screened for their cytotoxic activities against two murine cancer cell lines, L and 3LL, using the tetrazolium assay. While Amanitales and Russulales tested were not found active, Polyporales and Boletales gave better results. Four species exhibited a significant cytotoxic activity IC50 Suillus granulatus, S. The last one had never been investigated for its cytotoxic compounds before.

    Full Text Available In this study, grape seeds were extracted using ethyl acetate and petroleum ether by solvent-solvent extraction method. The phytochemical tests were performed to identify different phytochemical compounds present in the grape seed extract GSE. Antibacterial activity of the GSE was determined using agar diffusion method against Gram- positive and Gram-negative bacteria.

    Gas chromatography-mass spectrometry GC-MS and Fourier transform infrared spectroscopy FTIR analysis was done to identify the presence of bioactive compounds and their functional groups. The GC-MS results revealed a total of four compounds, known to have potent activity against cancer cells , viz, squalene, the most potent compound found in ethyl acetate extract and diethyl phthalate, ethyl cis trans octadecadienoate and R- ,-methylHexadecynol in petroleum ether extract.

    Cytotoxic activity of the GSE was observed against skin cancer cell lines A using 3- 4, 5-dimethylthiazolyldiphenyl tetrazolium bromide MTT assay. This is first such report against A cell lines. The study gives the overall perception about importance of GSE in medicine and nutraceuticals purposes. Full Text Available Endometrial cancer is a common malignancy of the female genital tract.

    This study demonstrates that Siegesbeckia orientalis ethanol extract SOE significantly inhibited the proliferation of RL human endometrial cancer cells. Treatment with SOE increased protein expression of caspase-3, -8 and -9 dose-dependently, indicating that apoptosis was through the intrinsic and extrinsic apoptotic pathways. In total, 10 constituents of SOE were identified by Gas chromatography-mass analysis.

    Caryophyllene oxide and caryophyllene are largely responsible for most cytotoxic activity of SOE against RL cells. Overall, this study suggests that SOE is a promising anticancer agent for treating endometrial cancer. Justicia gendarussa methanolic leaf extracts from five different locations in the Southern region of Peninsular Malaysia and two flavonoids, kaempferol and naringenin, were tested for cytotoxic activity.

    Kaempferol and naringenin were two flavonoids detected in leaf extracts using gas chromatography-flame ionization detection GC-FID. The results indicated that highest concentrations of kaempferol and naringenin were detected in leaves extracted from Mersing with Positive correlations were observed between kaempferol and naringenin concentrations in all leaf extracts analysed with the Pearson method.

    The extract of ginger Zingiber officinale Roscoe and its major pungent components, [6]-shogaol and [6]-gingerol, have been shown to have an anti-proliferative effect on several tumor cell lines. However, the anticancer activity of the ginger extract in pancreatic cancer is poorly understood. Here, we demonstrate that the ethanol- extracted materials of ginger suppressed cell cycle progression and consequently induced the death of human pancreatic cancer cell lines, including Panc-1 cells.

    The underlying mechanism entailed autosis, a recently characterized form of cell death, but not apoptosis or necroptosis. The autophagy inhibitors 3-methyladenine and chloroquine partially prevented cell death. Morphologically, however, focal membrane rupture, nuclear shrinkage, focal swelling of the perinuclear space and electron dense mitochondria, which are unique morphological features of autosis, were observed. The extract enhanced reactive oxygen species ROS generation, and the antioxidant N-acetylcystein attenuated cell death.

    Full Text Available The extract of ginger Zingiber officinale Roscoe and its major pungent components, [6]-shogaol and [6]-gingerol, have been shown to have an anti-proliferative effect on several tumor cell lines.

    Cytotoxic effects of Mangifera indica L. Waterlily Mango Mangifera indica L. Cytological studies on the breast cancer cells were also conducted, and phytochemical analyses of the extract were carried out to determine the likely bioactive compounds responsible for such effects. The extract showed significant toxicity towards both cell lines, with low toxicity to normal breast cells MCFA.

    The cytotoxic effects on the cells were further confirmed by the NR uptake, antiproliferative and LDH release assays. Bioactive analyses revealed that many bioactives were present in the extract although butylated hydroxytoluene, a potent antioxidant, was the most abundant with Synergistic effects of its antioxidant bioactives could have contributed to the cytotoxic effects of the extract.

    The extract of M. This potential is worth studying further, and could have implications on future studies and eventually management of human breast cancers. Full Text Available The Thelephora ganbajun mushroom has been found to be a potential rich source of natural antioxidants. Furthermore, the extracts obtained under the optimized conditions exhibited antiproliferative activities toward human lung A, breast MCF-7, liver HepG2 and colon HT cancer cells , especially for liver and lung cancer cells.

    Anti-proliferative effects of Bifidobacterium adolescentis SPM extract on human colon cancer cell lines. Lactic acid bacteria LAB are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as anti-tumor activity. The aim of the present work was to study the growth inhibition of tumor cells by butanol extract of Bifidobacterium adolescentis isolated from healthy young Koreans.

    The anti-proliferative activity of B. The effects of B. The butanol extract of B. Additionally, the butanol extract of B. Full Text Available Huaier extract is attracting increased attention due to its biological activities, including antitumor, anti-parasite and immunomodulatory effects.

    Huaier treatment inhibited cell viability in all three cell lines and induced various large membranous vacuoles in the cytoplasm. In addition, electron microscopy, MDC staining, accumulated expression of autophagy markers and flow cytometry revealed that Huaier extract triggered autophagy. Inhibition of autophagy attenuated Huaier-induced cell death. Cymbopogon citratus and Camellia sinensis extracts selectively induce apoptosis in cancer cells and reduce growth of lymphoma xenografts in vivo.

    Cancer cells are reported to have elevated levels of reactive oxygen species ROS and are highly dependent on cellular defense mechanisms against oxidative stress. Numerous nutraceuticals and natural polyphenolic compounds have a wide range of abilities to alter cellular redox states with potential implications in various diseases.

    Furthermore, therapeutic options for cancers are mostly nonselective treatments including genotoxic or tubulin-targeting compounds. Some of the natural extracts , containing multiple bioactive compounds, could target multiple pathways in cancer cells to selectively induce cell death. Cymbopogon citratus lemongrass and Camellia sinensis white tea extracts have been shown to have medicinal properties, however, their activity against lymphoma and leukemia, as well as mechanistic details, have not been fully characterized.

    Herein, we report potent anti- cancer properties in dose and time-dependent manners of ethanolic lemongrass and hot water white tea extracts in lymphoma and leukemia models.

    Both extracts were able to effectively induce apoptosis selectively in these human cancer cell types. Interestingly, ethanolic lemongrass extract induces apoptosis primarily by the extrinsic pathway and was found to be dependent on the generation of ROS.

    Conversely, apoptotic induction by hot water white tea extract was independent of ROS. Furthermore, both of these extracts caused mitochondrial depolarization and decreased rates of oxygen consumption in lymphoma and leukemia cells , leading to cell death.

    Most importantly, both these extracts were effective in reducing tumor growth in human lymphoma xenograft models when administered orally. Thus, these natural extracts could have potential for being nontoxic alternatives for the treatment of cancer. Green tea extract selectively targets nanomechanics of live metastatic cancer cells. Cancer 9 with various biological activities Lu et al Clin. The biomechanical response of GTE treated cells taken directly from patient's body samples was measured using atomic force microscopy AFM Binnig et al Phys.

    We found significant increase in stiffness of GTE treated metastatic tumor cells , with a resulting value similar to untreated normal mesothelial cells , whereas mesothelial cell stiffness after GTE treatment is unchanged.

    Immunofluorescence analysis showed an increase in cytoskeletal-F-actin in GTE treated tumor cells , suggesting GTE treated tumor cells display mechanical, structural and morphological features similar to normal cells , which appears to be mediated by annexin-I expression, as determined by siRNA analysis of an in vitro cell line model. Our data indicates that GTE selectively targets human metastatic cancer cells but not normal mesothelial cells , a finding that is significantly advantageous compared to conventional chemotherapy agents.

    Pro-apoptotic and anti-proliferative effects of corn silk extract on human colon cancer cell lines. Corn silk is an economically and nutritionally significant natural product as it represents a staple food for a large proportion of the world population. This study investigated the anticancer activity of corn silk extract in human colon cancer cells and human gastric cancer cells. Following treatment with corn silk extract , certain apoptosis-related events were observed, including inhibition of cell proliferation, loss of mitochondrial membrane potential??

    Antimetastatic effects of Rheum palmatum L. However, the detailed impacts and underlying mechanisms of R. Gelatin zymography, Western blot, real-time polymerase chain reaction, and luciferase assay were used to explore the underlying mechanisms involved in the antimetastatic effects on oral cancer cells.

    Thus, RLEs may be potentially useful as antimetastatic agents for oral cancer chemotherapy. Effects of different extracts of curcumin on TPC1 papillary thyroid cancer cell line.

    The thyroid gland is one of the largest endocrine glands in the body. Curcumin possesses a wide variety of biological functions, and thanks to its properties, it has gained considerable attention due to its profound medicinal values Prasad, Gupta, Tyagi, and Aggarwal, Biotechnol Adv We have undertaken the present work in order to define the possible role of curcumin in modulating the genetic expression of cell markers and to understand the effectiveness of this nutraceutical in modulating the regression of cancer phenotype.

    As a template we used the TPC-1 cells treated with the different extracts of turmeric, and examined the levels of expression of different markers proliferative, inflammatory, antioxidant, apoptotic.

    Treatment with the three different curcumin extracts displays anti-inflammatory, antioxidant properties and it is able to influence cell cycle with slightly different effects upon the extracts. Furthermore curcumin is able to influence cell metabolic activity vitality. In conclusion curcumin has the potential to be developed as a safe therapeutic but further studies are needed to verify its antitumor ability in vivo. Our results revealed that corn silk extract inhibited the proliferation of cancer cells and increased the level of apoptosis in a concentration-dependent manner.

    Western blot analysis revealed that corn silk extract upregulated the levels of Bax, cytochrome c , caspase-3 and caspase-9, but downregulated the levels of B- cell lymphoma 2. These results suggest that corn silk extract may induce apoptosis through the mitochondria-mediated pathway.

    Full Text Available Type II endometrial carcinoma typically exhibits aggressive metastasis and results in a poor prognosis. Siegesbeckia orientalis Linne is a traditional Chinese medicinal herb with several medicinal benefits, including the cytotoxicity against various cancers. This study investigates the inhibitory effects of S. The inhibitory effects were evaluated by determining wound healing and performing the Boyden chamber assay.

    The results of this investigation suggest that SOE is a potential anti-metastatic agent against human endometrial tumors. Green tea extract induces protective autophagy in A non-small lung cancer cell line. For many decades, polyphenols, including green tea extract catechins, have been reported to exert multiple anti-tumor activities.

    However, to date the mechanisms of their action have not been completely elucidated. Thus, the aim of this study was to assess the effect of green tea extract on non-small lung cancer A cells. A cells following treatment with GTE were analyzed using the inverted light and fluorescence microscope. In order to evaluate cell sensitivity and cell death, the MTT assay and Tali image-based cytometer were used, respectively.

    Ultrastructural alterations were assessed using a transmission electron microscope. Likewise, the treatment with GTE resulted in only a very small dose-dependent increase in the population of apoptotic cells. However, enhanced accumulation of vacuole-like structures in response to GTE was seen at the light and electron microscopic level.

    Furthermore, an increase in the acidic vesicular organelles and LC3-II puncta formation was observed under the fluorescence microscope, following GTE treatment.

    The analysis of the functional status of autophagy revealed that GTE-induced autophagy may provide self-protection against its own cytotoxicity, since we observed that the blockage of autophagy by bafilomycin A1 decreased the viability of A cells and potentiated necrotic cell death induction in response to GTE treatment.

    Collectively, our results revealed that A cells are insensitive to both low and high concentrations of the green tea extract , probably due to the induction of cytoprotective autophagy. These data suggest that a potential utility of GTE in lung cancer therapy may lie in its synergistic combinations with drugs or small molecules that target autophagy, rather than in monotherapy. Full Text Available Background and objectives: Selective modulation of endoplasmic reticulum stress markers in prostate cancer cells by a standardized mangosteen fruit extract.

    Full Text Available The increased proliferation of cancer cells is directly dependent on the increased activity of the endoplasmic reticulum ER machinery which is responsible for protein folding, assembly, and transport. In fact, it is so critical that perturbations in the endoplasmic reticulum can lead to apoptosis.

    This carefully regulated organelle represents a unique target of cancer cells while sparing healthy cells. In this study, a standardized mangosteen fruit extract MFE was evaluated for modulating ER stress proteins in prostate cancer. Next, we evaluated MFE for microsomal stability and anti- cancer activity in nude mice.

    MFE induced apoptosis, decreased viability and proliferation in prostate cancer cells. MFE increased the expression of ER stress proteins.

    MFE suppressed tumor growth in a xenograft tumor model without obvious toxicity. Mangosteen fruit extract selectively promotes endoplasmic reticulum stress in cancer cells while sparing non-tumorigenic prostate epithelial cells. Furthermore, in an in vivo setting mangosteen fruit extract significantly reduces xenograft tumor formation.

    The increased proliferation of cancer cells is directly dependent on the increased activity of the endoplasmic reticulum ER machinery which is responsible for protein folding, assembly, and transport. The extract from Punica granatum pomegranate peel induces apoptosis and impairs metastasis in prostate cancer cells. Prostate cancer is a big threat to male for its poor prognosis and high mortality rate.

    Natural compounds are important resources of many anticancer drugs. Pomegranate is a kind of antioxidant-rich fruit and its peel and seed has potential anticancer activities. In this study, we aimed to investigate the effects of pomegranate peel extract PoPx on the apoptosis and metastasis of prostate cancer cells and the related mechanism. We found that PoPx showed growth inhibition on prostate cancer cells. Nuclei morphological and flow cytometer FCM analysis indicated that PoPx could induce prostate cancer apoptosis.

    Further investigation indicated that mitochondrial mediated intrinsic pathway is involved in the apoptosis. Wound healing assay and transwell migration and invasion assay implied that PoPx has the potential to inhibit migration and invasion, two critical steps in prostate cancer metastasis. In summary, the present data showed that PoPx could be a promising drug candidate to treat prostate cancer , showing us a better way to develop novel drugs from natural compounds.

    Due to the chemo resistant nature of cancer cells and adverse effects of current therapies, researchers are looking for the most efficient therapeutic approach which has the lowest side effects and the highest toxicity on cancer cells. The aim of the present study was to investigate the synergic effect of Urtica dioica extract in combination with paclitaxel on cell death and invasion of human breast cancer MDA-MB cell line.

    To determine the cytotoxic effects of Urtica dioica extract with paclitaxel, MTT assay was performed. The scratch test was exploited to assess the effects of Urtica dioica, Paclitaxel alone and combination on migration of cancer cells. The effects of plant extract , Paclitaxel alone and combination on different phases of cell cycle was analyzed using flow cytometry.

    Results of MTT assay showed that Urtica dioica significantly destroyed cancer cells. Interestingly, Concurrent use of Urtica dioica extract with paclitaxel resulted in decreased IC50 dose of paclitaxel.

    Moreover, findings of scratch assay exhibited the inhibitory effects of Urtica dioica, Paclitaxel alone and combination on migration of MDA-MB cell line. Our findings also demonstrated that the extract substantially decreased the Snail-1 and related gene expression.

    Our results demonstrated that the dichloromethane extract of Urtica dioica inhibit cell growth and migration. Also, Urtica dioica extract substantially increased sensitivity of breast cancer cells to paclitaxel. Therefore, it can be used as a potential candidate for treatment of breast cancer with paclitaxel. When the cytokine TNF-[Formula: As a result, the active NF-[Formula: Bax subfamily proteins induced apoptosis through caspase Imperata cylindrica, a tall tufted grass which has multiple pharmacological applications is one of the key ingredients in various traditional medicinal formula used in India.

    Previous reports have shown that I. To our knowledge, no studies have been published on the effect of I. The present study was undertaken in order to evaluate the anticancer properties of the leaf extract of I. A methanol extract from dried leaves of I. Cytotoxicity was determined by MTT assay. Cell cycle analysis was performed by flow cytometry and induction of apoptosis was determined by DNA fragmentation assay.

    The ICL extract treatment caused cytotoxicity and induced cell death in vitro in SCC-9 cells in a dose-dependent manner. Furthermore, DNA fragmentation assays showed that the observed cell death was caused by apoptosis.

    This is the first report showing the anticancer activity of the methanol extracts from the leaves of I. The natural abundance of I. Effect of crude saponins from Gaultheria trichophylla extract on growth inhibition in human colorectal cancer cells. Full Text Available The genus Gaultheria also comprised of species with reported cytotoxic activities. Current research work was carried out to evaluate G. Caco-2 cells treated with the crude extract showed significant growth inhibition p cell treated with crude saponins observed under confocal microscope showed shrunken nuclei with apparent nuclear fragmentation and chromatin condensation indicating apoptosis mode of cell death.

    The study exhibited that the G. Trichophylla saponins induced apoptosis of Caco-2 cell lines. This study provides new evidences to further explore this plant for the novel targets in anticancer drug development. The dual effects of polar methanolic extract of Hypericum perforatum L. PMF has been tested in human leukemic cells , HL cells , cord blood hemopoietic progenitor cells , bladder cancers derived from metastatic lymph node T and primary papillary bladder lesion RT However, the mechanisms of the effects of PMF on these human cell lines have not been elucidated.

    PMF was prepared from an aerial herb of HPL which was brewed in methanol and extracted with ether and methanol. Isolated DNA samples were separated by electrophoresis in 1.

    The initial cell cycle analysis and phase distribution was by flow cytometry. DNA synthesis was measured by [3H] thymidine incorporation, and cell cycle regulatory proteins were assayed by Western immunoblot. Effects of Calophyllum inophyllum fruit extract on the proliferation and morphological characteristics of human breast cancer cells MCF Full Text Available Objective: To evaluate the antiproliferative activity of Calophyllum inophyllum C. The cytotoxic effect of C.

    The preliminary time-based morphological investigation of MCF-7 cells treated with the IC 50 value This study clearly proved that the proliferation of human breast cancer cell MCF-7 was inhibited by C. Research on marine natural products as potential anticancer agents is still limited.

    In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers , including lung, prostate, stomach, breast, and pancreatic cancers , as well as an osteosarcoma.

    In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells grown in suspension than to adherent cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells.

    Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted. Full Text Available Research on marine natural products as potential anticancer agents is still limited. Antiproliferative effects of the readily extractable fractions prepared from various citrus juices on several cancer cell lines.

    Screening of 34 Citrus juices indicated that King Citrus nobilis strongly inhibited proliferation of all cancer cell lines examined. Sweet lime and Kabuchi inhibited three of the four cancer cell lines. In contrast, these samples were substantially less cytotoxic toward normal human cell lines. Supercritical carbon dioxide extract of Physalis peruviana induced cell cycle arrest and apoptosis in human lung cancer H cells.

    PP is a popular folk medicine used for treating cancer , leukemia, hepatitis, rheumatism and other diseases. In this study, our objectives were to examine the total flavonoid and phenol content of different PP extracts aqueous: EEPP; supercritical carbon dioxide: Among all the extracts tested, results showed that SCEPP-5 possessed the highest total flavonoid SCEPP-5 not only induced cell cycle arrest at S phase, it also up-regulated the expression of pro-apoptotic protein Bax and down-regulated the inhibitor of apoptosis protein IAP.

    Furthermore, the apoptotic induction in H cells was found to associate with an elevated p53 protein expression, cytochrome c release, caspase-3 activation and PARP cleavage. Taken together, these results conclude that SCEPP-5 induced cell cycle arrest at S phase, and its apoptotic induction could be mediated through the pdependent pathway and modification of Bax and XIAP proteins expression.

    The results have also provided important pharmacological backgrounds for the potential use of PP supercritical fluid extract as products for cancer prevention. Antiproliferative and apoptotic effect of Morus nigra extract on human prostate cancer cells. Numerous studies have investigated the antiproliferative effects of various extracts of different Morus species, but studies involving the in vitro cytotoxic effect of M. The purpose of this study was to evaluate the phenolic composition and antioxidant activity of dimethyl sulfoxide extract of M.

    Mechanisms involved in the cytotoxic effect of DEM on PC-3 cells were then investigated in terms of apoptosis, mitochondrial membrane potential and cell cycle using flow cytometry, while caspase activity was investigated using luminometric analysis. Ascorbic acid and chlorogenic acid were the major phenolic compounds detected at HPLC analysis. DEM arrested the cell cycle of PC-3 cells at the G1 phase, induced apoptosis via increased caspase activity and reduced mitochondrial membrane potential.

    Our results indicate that M. The study was conducted to investigate the anticancer potential of different parts of G. MTT assay showed that the peel, flesh, and seed extracts of G.

    The flesh extract of G. Caspase-3 has been shown to be activated which finally leads to the death of HepG2 cell apoptosis. GC-MS analysis showed that the highest percentage of compound identified in the extract of G. This finding suggested that the flesh extract of G. Full Text Available Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient.

    In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Tapirira guianensis Aubl. Analysis of secondary metabolites classes in fractions of T. Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability.

    Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation.

    Antiproliferative activity of T. Bullock's-heart Annonaceae family is a semi-evergreen and small deciduous tree. The extracts of various parts of Annona reticulata L. Detection of phosphatidylserine on membranes of apoptotic cells was done by Attune flow cytometer.

    The Paclitaxel was used as a control standard drug for the study. The downregulation of Bcl-2 and upregulation of Bax and Bak, and caspases activation suggested induction of apoptosis in TD cells by ARME through mitochondrial pathway. Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells.

    Ethnotraditional use of plant-derived natural products plays a significant role in the discovery and development of potential medicinal agents. Plants of the genus Taraxacum, commonly known as dandelions, have a history of use in Chinese, Arabian and Native American traditional medicine, to treat a variety of diseases including cancer.

    To date, however, very few studies have been reported on the anti-carcinogenic activity of Taraxacum officinale TO.

    In the present study, three aqueous extracts were prepared from the mature leaves, flowers and roots, and investigated on tumor progression related processes such as proliferation and invasion. Inhibition of invasion was further evidenced by decreased phosphorylation levels of FAK and src as well as reduced activities of matrix metalloproteinases, MMP-2 and MMP This study provides new scientific data on TO and suggests that TO extracts or individual components present in the extracts may be of value as novel anti- cancer agents.

    Cytotoxic, antimigratory, pro-and antioxidative activities of extracts from medicinal mushrooms on colon cancer cell lines.

    Full Text Available Methanol extracts of five commercially available mushroom species Phellinus linteus Berk. Pegler, Coprinus comatus O. Spectrophotometric methods were used for the determination of total phenol content, flavonoid concentrations and DPPH activity of the extracts. Cytotoxic activity was measured by the MTT assay.

    Our results show that the highest phenolic and flavonoid content was found in P. All other extracts selectively inhibited SW cell viability, but did not show significant cytotoxic activity.

    The results of the present research indicate that the mushroom extracts causes oxidative stress which has a pronounced impact on the migratory status of colon cancer cell lines.

    Anticancer potential of Conium maculatum extract against cancer cells in vitro: Conium maculatum extract is used as a traditional medicine for cervix carcinoma including homeopathy.

    However, no systematic work has so far been carried out to test its anti- cancer potential against cervix cancer cells in vitro.

    Thus, in this study, we investigated whether ethanolic extract of conium is capable of inducing cytotoxicity in different normal and cancer cell lines including an elaborate study in HeLa cells. Conium's effects on cell cycle, reacti Full Text Available Herbal medicines are often combinations of botanical extracts that are assumed to have additive or synergistic effects. The purpose of this investigation was to compare the effect of individual botanical extracts with combinations of extracts on prostate cell viability.

    We then modeled the interactions between botanical extracts in combination isobolographically. Scutellaria baicalensis, Rabdosia rubescens, Panax-pseudo ginseng, Dendranthema morifolium, Glycyrrhiza uralensis and Serenoa repens were collected, taxonomically identified and extracts prepared.

    Effects of the extracts on cell viability were quantitated in prostate cell lines using a luminescent ATP cell viability assay. Combinations of two botanical extracts of the four most active extracts were tested in the 22Rv1 cell line and their interactions assessed using isobolographic analysis. Each extract significantly inhibited the proliferation of prostate cell lines in a time- and dose-dependent manner except repens. The most active extracts , baicalensis, D.

    The remaining two- extract combinations showed antagonism. The four extracts together were significantly more effective than the two-by-two combinations and the individual extracts alone. Combining the four herbal extracts significantly enhanced their activity in the cell lines tested compared with extracts alone.

    The less predictable nature of the two-way combinations suggests a need for careful characterization of the effects of each individual herb based on their intended use. Scholtysek, Carina; Krukiewicz, Aleksandra A. An assumption is that SPBE affects tumor cell progression and migration in breast and prostate tissue.

    In the presence of cholesterol, these features are not only reversed but increased significantly. Since the protein p53 is also regarded as nuclear matrix protein facilitating actin cytoskeletal binding, 2D tractions were measured.

    The results suggest a dual function of p53 in cells. In this work, DU cells were used to demonstrate that SPBE and its sterol components, beta-sitosterol and stigmasterol, inhibit prostate cancer growth by increasing p53 protein expression and also inhibit carcinoma development by decreasing p21 and p27 protein expression. The results show for the first time the potential of SPBE, beta-sitosterol and stigmasterol as potential anti-tumor agents.

    The cell adhesion strength in the presence of SPBE, beta-sitosterol and cholesterol and the observation was that the increase in p53 expression triggered an increase in the intracellular force generation. Anticancer activity of Kalanchoe tubiflora extract against human lung cancer cells in vitro and in vivo.

    Uncontrolled cell proliferation is a common feature of human cancer. Some of herbal extract or plant-derived medicine had been shown as an important source of effective anticancer agents. We previously reported that an n-BuOH-soluble fraction of Kalanchoe tubiflora has antiproliferative activity by inducing mitotic catastrophe.

    In this study, we showed that the H 2 O-soluble fraction of Kalanchoe tubiflora KT-W caused cell cycle arrest, and senescence-inducing activities in A cells. We used 2 dimensional PAGE to analyze the protein expression levels after KT-W treatment, and identified that the energy metabolism-related proteins and senescence-related proteins were disturbed. In vivo experiments showed that the tumor growths in Axenografted nude mice were effectively inhibited by KT-W.

    Our findings implied that KT-W is a putative antitumor agent by inducing cell cycle arrest and senescence. Phytochemical properties and anti-proliferative activity of Olea europaea L. Oleuropein has been shown to exhibit anti-proliferative activity against a number of cancer types. However, they have not been tested against pancreatic cancer , the fifth leading cause of cancer related death in Western countries.

    For this reason, olive leaf extracts warrant further investigation into their potential anti-pancreatic cancer benefits.

    Full Text Available The methanol extracts of the aerial part of four ethnomedicinal plants of Mediterranean region, two non-seed vascular plants, Equisetum hyemale L.

    Newman, and two Spermatophyta, Juniperus communis L. Only the extracts of J. A dose-dependent effect of these two extracts was also observed on the cell cycle distribution.

    Incubation of all the cell lines in a medium containing J. The novelty of our findings stands on the observation that the two extracts , consistently, elicited coherent effects on the cell cycle in four cell lines, independently from their phenotype, as two of them have epithelial origin and grow adherent and two are lymphoblastoid and grow in suspension.

    Even the expression profiles of several proteins regulating cell cycle progression and cell death were affected by both extracts. LC-MS investigation of methanol extract of C. Although many of these compounds have interesting individual biological activities, their natural blends seem to exert specific effects on the proliferation of cell lines either growing adherent or in suspension, suggesting potential use in fighting cancer.

    Biological activity of Xanthium strumarium seed extracts on different cancer cell lines and Aedes caspius, Culex pipiens Diptera: Full Text Available Effects of methanol extracts of Xanthium strumarium on different cancer cell lines and on the mortality rates of Aedes caspius, Culex pipiens Diptera: Among the cell lines tested, the Jurkat cell line was the most sensitive to the methanol extract and ethyl acetate fraction, with reported LC50 values of Conversely, methanol extracts were not that toxic to the A cell line though the toxicity increased on further purification.

    The percentage of growth inhibition was dose dependent for the methanol extract and ethyl acetate fraction. The ethyl acetate fraction showed higher toxicity to all cell lines tested when compared to the methanol extract. From the investigations, it was concluded that the crude extract of X. However, on further purification the extract lost the larvicidal activity.

    The ethyl acetate fraction investigated in this study appears to have a weak larvicidal activity but a promising cytotoxic activity. Future studies will include purification and investigation in further detail of the action of X.

    Effects of methanol extracts of Xanthium strumarium on different cancer cell lines and on the mortality rates of Aedes caspius, Culex pipiens Diptera: Among the cell lines tested, the Jurkat cell line was the most sensitive to the methanol extract and ethyl acetate fraction, with reported LC 50 values of Anti- cancer potential of a mix of natural extracts of turmeric, ginger and garlic: A cell -based study. Full Text Available Cancer related morbidity and mortality is a major health care concern.

    Developing potent anti- cancer therapies which are non-toxic, sustainable and affordable is of alternative medicine. This study was designed to investigate the aqueous natural extracts mixture NE mix prepared from common spices turmeric, ginger and garlic for its free radical scavenging potential and anti- cancer property against human breast cancer cell lines MCF-7, ZR and MDA-MB To the best of our knowledge, NE mix with and without Tamoxifen has not been tested for its anti- cancer potential.

    The extent of apoptosis due to combined treatment with NE mix-Tamoxifen was higher than Tamoxifen alone, indicating a potential role of the NE mix in sensitizing the ER-positive breast cancer cells towards Tamoxifen. Our data strongly indicate that our NE mixture is a potential alternative therapeutic approach in certain types of cancer. The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells.

    Despite a lack of scientific authentication, Scutellaria baicalensis is clinically used in Chinese medicine as a traditional adjuvant to chemotherapy of lung cancer. In this study, cytotoxicity assays demonstrated that crude ethanolic extracts of S. The active compounds baicalin, baicalein and wogonin did not exhibit such selectivity. The altered protein expression indicated that cell growth arrest and apoptosis were potential mechanisms of cytotoxicity.

    These observations were supported by PI staining cell cycle analysis using flow cytometry and Annexin-V apoptotic analysis by fluorescence microscopy of cancer cells treated with the crude extract and pure active compounds. Following treatment, increased expression in the cancer cells of key proteins related to the enhancement of apoptosis was observed for p53 and Bax. These results provide further insight into the molecular mechanisms underlying the clinical use of this herb as an adjuvant to lung cancer therapy.

    Portulaca oleracea extract can inhibit nodule formation of colon cancer stem cells by regulating gene expression of the Notch signal transduction pathway. To investigate whether Portulaca oleracea extract affects tumor formation in colon cancer stem cells and its chemotherapy sensitivity.

    In addition, to analyze associated genetic changes within the Notch signal transduction pathway. Serum-free cultures of colon cancer cells HT and HT cancer stem cells were treated with the chemotherapeutic drug 5-fluorouracil to assess sensitivity.

    In addition, the effect of different concentrations of P. The effects of P. The tumor volume of the HT29 cells was two times larger than that of HT29 cancer stem cells.

    Apoptosis of HT cancer cells and HT cancer stem cells was assessed by flow cytometry; it was enhanced by the addition of P. Finally, treatment with P. The results of this study show that P.

    Taken together, this suggests that it may elicit its effects through regulatory and target genes that mediate the Notch signal transduction pathway. Avicennia marina AM is a widely distributed mangrove plant that has been used in traditional medicine for centuries for the treatment of a number of diseases. The objective of the present study was to evaluate the leaf ethyl acetate extract of AM for its cytotoxic and apoptotic potential along with in-depth investigations of its mechanism of action in breast cancer MCF-7 cells.

    The ethyl acetate extract of leaves and stems of AM was tested against estrogen positive breast cancer cell line MCF-7 using various assays. Place and Duration of Study: Dose- and time-dependent growth inhibition of cancer cells was measured using MTT assay. The mechanisms of apoptosis induction were determined using various assays: The AM extract inhibited breast cancer cell growth and induced apoptosis in a concentration dependent manner.

    We demonstrated a non-classical mode of apoptosis induction in MCF-7 cells by AM extract , where ROS production altered the mitochondrial membrane potential to induce apoptosis. A significant amount of autophagy was also observed at the same concentration. Inhibitory activities of Perilla frutescens britton leaf extract against the growth, migration, and adhesion of human cancer cells. Cancer is a major cause of human death worldwide.

    The aim of the current study was to investigate the inhibitory effects of ethanol extract of perilla leaf PLE against important characteristics of cancer cells , including unrestricted growth, resisted apoptosis, and activated metastasis, using human cancer cells.

    Assays using 3- 4,5-dimethylthiazolyl -2,5-diphenyltetrazolium bromide were performed for measurement of cell growth. Soft agar and wound healing assays were performed to determine colony formation and cell migration, respectively. Nuclear staining and cell cycle analysis were performed for assessment of apoptosis.

    Fibronectin-coated plates were used to determine cell adhesion. PLE at the concentration range of Further studies are needed in order to determine whether similar effects are reproduced in vivo. Differences of response of human bladder cancer cells to photodynamic therapy PDT with Hypericum perforantum L extract and Photofrin. We have reported on the antiproliferative activity of the lipophilic extract of the Hypericum perforatum L.

    The PMF was extracted from the dry herb with methanol, followed by liquid extraction with petroleum ether. After 24 hours, the cells were treated with laser light nm with 0,1,2,4 and 8 Joules. The cells were then washed and reincubated for another 24 hours. After this incubation cell survival was assessed by the MTT assay. Understanding mechanisms of such differential responses might prove useful. Alpinia scabra, locally known as 'Lengkuas raya', is an aromatic, perennial and rhizomatous herb from the family Zingiberaceae.

    It is a wild species which grows largely on mountains at moderate elevations in Peninsular Malaysia, but it can also survive in the lowlands like in the states of Terengganu and Northern Johor. The present study reports the cytotoxic potential of A. The experimental approach in the present study was based on a bioassay-guided fractionation.

    The crude methanol and fractionated extracts hexane, chloroform and water from different parts of A. The identified cytotoxic extracts were then subjected to chemical investigations in order to identify the active ingredients. A normal human lung fibroblast cell line MRC-5 was used to determine the specificity for cancerous cells. The cytotoxic extracts and fractions were also subjected to morphological assessment, DNA fragmentation analysis and DAPI nuclear staining.

    The leaf hexane and chloroform and rhizome chloroform extracts showed high inhibitory effect against the tested cells. Ten fractions LC1-LC10 were yielded after purification of the leaf chloroform extract.

    Meanwhile, eighteen fractions RC1-RC18 were obtained after purification of the rhizome chloroform extract , of which fraction RC5 showed cytotoxicity against SKOV-3 cells with high selectivity index. Aim of the work: Animal urine, including that of camels, has long been used for the therapeutic management of human ailments. In this study, we sought to characterize the cytotoxic properties of newly derived purified fractions from previously described camel urine extract PMF on various cancer cell lines.

    This study showed that the newly derived and more purified fraction of PMF new PMF possesses effective and selective anti- cancer properties against several types of cancer cell lines. This study, as well as previous ones, suggests that camel urine extracts old and new PMF may provide newer therapeutic alternatives to clinically manage cancer patients.

    However, further studies are needed to verify these positive preliminary results. Moringa Oleifera aqueous leaf extract down-regulates nuclear factor-kappaB and increases cytotoxic effect of chemotherapy in pancreatic cancer cells.

    Chemotherapy is currently the standard treatment, however, these tumors often develop drug resistance over time. Agents for increasing the cytotoxic effects of chemotherapy or reducing the cancer cells ' chemo-resistance to the drugs are required to improve treatment outcome.

    Nuclear factor kappa B NF-kB , a pro-inflammatory transcription factor, reportedly plays a significant role in the resistance of pancreatic cancer cells to apoptosis-based chemotherapy. This study investigated the effect of aqueous Moringa Oleifera leaf extract on cultured human pancreatic cancer cells - Panc-1, p34, and COLO , and whether it can potentiates the effect of cisplatin chemotherapy on these cells.

    The effect of Moringa Oleifera leaf extract alone and in combination with cisplatin on the survival of cultured human pancreatic cancer cells was evaluated by XTT-based colorimetric assay. The distribution of Panc-1 cells in the cell cycle following treatment with Moringa leaf extract was evaluated by flow cytometry, and evaluations of protein levels were via immunoblotting.

    Data of cell survival following combined treatments were analyzed with Calcusyn software. Moringa Oleifera leaf extract inhibited the growth of all pancreatic cell lines tested. Lastly, Moringa Oleifera leaf extract synergistically enhanced the cytotoxic effect of cisplatin on Panc-1 cells. Herbal tea extract combined with light-induced significant in vitro cytotoxicity of human bladder cancer cells.

    The anti-inflammatory, anti-microbial, antiviral, and antidepressant activities of the Greek herb, Hypericum Perforatum L, HP L, have been attributed to the total extract or single constituents. The PMF was extracted from the dry herb with methanol, followed by liquid-liquid extraction with petroleum ether. After 24 hours, the cells were subjected to laser light nm treatment with 0, 1, 4 and 8 Joules.

    Our data suggest that MPF may be an effective agent for in vitro photodynamic therapy. Confirmation of these results in preclinical studies may lead to clinical trials.

    Full Text Available In Indian traditional medicine, Boerhaavia diffusa punarnava roots have been widely used for the treatment of dyspepsia, jaundice, enlargement of spleen, abdominal pain and as an anti-stress agent.

    Pharmacological evaluation of the crude ethanolic extract of B. The extract of B.

    Effect of Cannabis sativa crude extracts on caspase 3/7 activity Cannabidiol reduces cell viability of cervical cancer cells .. Figure Caspase 3/7 activity after treatment of SiHa, HeLa, and ME cells with IC50 of . Cannabidiol, extracted from Cannabis sativa, selectively inhibits .. (– mg /kg), Δ5-avenasterol (89– mg/kg) and stigmasterol (75– Selective inhibition of deactivated mitochondrial complex I by biguanides. no effect on the decrease in cell volume or the increase in caspase-3/7 activity induced by TNFα. Cannabidiol rather than Cannabis sativa extracts inhibit cell growth and morphological changes, an increase in Caspase 3/7 and a decrease in the . Kaempferia parviflora Extract Exhibits Anti-cancer Activity against HeLa Cervical Cancer Cells. Apoptotic Effect of the Urtica Dioica Plant Extracts on Breast Cancer Cell.

    Associated Data



    Effect of Cannabis sativa crude extracts on caspase 3/7 activity Cannabidiol reduces cell viability of cervical cancer cells .. Figure Caspase 3/7 activity after treatment of SiHa, HeLa, and ME cells with IC50 of .


    Cannabidiol, extracted from Cannabis sativa, selectively inhibits .. (– mg /kg), Δ5-avenasterol (89– mg/kg) and stigmasterol (75– Selective inhibition of deactivated mitochondrial complex I by biguanides. no effect on the decrease in cell volume or the increase in caspase-3/7 activity induced by TNFα.


    Cannabidiol rather than Cannabis sativa extracts inhibit cell growth and morphological changes, an increase in Caspase 3/7 and a decrease in the . Kaempferia parviflora Extract Exhibits Anti-cancer Activity against HeLa Cervical Cancer Cells. Apoptotic Effect of the Urtica Dioica Plant Extracts on Breast Cancer Cell.


    ClC-3 chloride (Cl–) channel has been shown to be involved in cell proliferation, cell pathway, was involved in the antitumor activities of bufadienolides. .. figures showing effects of bufalin and 15β-acetyloxybufalin on caspase 3/7 table listing cytotoxicities of bufadienolides in cell lines H22 and S


    stomatitis from the drugs on days than those using the ordinary mouthwashes with saline, .. increasing the IC50 fold without impacting its antitumor activity on HeLa cells (in vitro) effect of diazepam by further decreasing motor activity. *Cannabis sativa or Cannabis indica leaves and tops.

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