Details of Indian Import Data of SomatropinDuring the past few years, more than products suspected to contain somatropin have been analysed. Some somatropin crs batch 3 the samples were also subjected to somatropin crs batch 3 microorganisms and endotoxins. The identification of somatropin was carried out by peptide mapping using btach as the proteolytic enzyme. A double chain peptide cross-linked via a disulfide bond was used as the signature peptide. Capillary electrophoresis in double injection mode was applied to both identification and purity determination of the samples. The identification was based on the comparison between the steroid cycle to lean out migration time of the reference standard and the calculated migration time of the analyte, being present in the second injection plug.
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Bodybuilding Forums - JuicedMuscle. As we reported before there where some busts in China http: I'll post some analyses and post the text as I got it: Total four kigtropin were tested, first kigtropin was non protein non GH product. Since i was not told to save it, i threw that one out. No need to keep illegal even if its fake stuff in the fridge that i wont sell or use. Riptropin SDS good gh no extra bands, very fat target band i am going to do BCA protein concentration this weekend if i have time.
No GH detected Identification by MW molecular weight and purity in protein samples, all reagents and equipment are calibrated and qualified. All samples reduced and heated. Biorad Precision marker 2. Biorad Precision marker 6. I explain denatured GH produces 10 and 7kDa naturally and its proven to be perfectly normal in literature.
Elitropin lane 1 and 2 are non-denatured and there is a size difference as it is more heavier than denatured so I will denature GH to test it as the target big fat band at 4 and 5 land right at kDa. I wouldnt think protein concentration would go down, probably degraded but total protein fractured or intact would be the same from the absorbance.
I would rather have 4iu correct sequenced GH than 15 iu with wrong aminoacids in the chain. Also, there could in theory be some additives in the vial that messes with the results and the additives is probably not the same between the products.
Lets save these results and we will figure out more when we have the results from germany. These samples are bought by a chinese middleman, from diverse sources and manufacturers. First to detect presence indentification by MW. Then he will sent the 4 best samples to our German Lab to analyse these samples complete also for toxins - dimers etc. The best he will buy in bulk for sell. We just assist him since we all know many guys are ripped or buy bunk.
Naps GH is great quality and purity. Comes from a reputable manufacturer that does not have product line anymore. There is much counterfeiting, but its important to know where you buy and start small amounts and try them study them and the boxes etc.
I agree with you RT. In fact after your analysis came out I purchased 3 kits of BT from that source and they are in fact, very good products.
I appreciate all the work you do keeping our sources honest. Who are you talking about that will buy in bulk and sell? I'm talking about a Chinese middleman that buys and sells bulk quantities of peptides. A few guys assist him with analyses, to ensure quality. Because I have access to a peptide lab he asked me to sent samples for analytical analysis.
I use the opportunity to inform you guys about the tricky market of today in China etc. I have had it with crooks.. I spoke to soon on my recommendation. The tested blue tops seem to be very inconsistent from vial to vials and from kit to kit. The vials in the front of th kit were very good. As I pulled vils from the back of the kit they were no good at all. The majority of the 3 kits I bought turned out to be crap. This source has since then quit selling blue top generics and has replaced my 3 kits with Hygetropin which is excellent front and back of the kit.
Even buying from a good reputable source is risky. Thank goodness this guy backs his products. All major Chinese brands are in the analytical lab who finaly received their C8 to enable potency testing. Naps had Hygetropins in stock!! Right up there with pharm brand. If they are please feel free to edit my post and repost etcAnd if there nothing to do with it ill edit my post.. The first results on Chinese Growth Hormone, once the graphics are in I'll post the full report and graphs here we have completed the tests of the four samples and are writing the report.
All samples gave very similar results as the Somatropin CRS, thus all samples really contain somatropin. For strength and related proteins, reversed-phase HPLC was performed according to the supplied standard testing procedure. Tthe strength has been calculated using the peak area of the main signal, which had the same retention time as the signal of the standard.
The peak area of the related proteins desamido-somatropin is given in percent of total peak area. Strength is given in mg per vial and i. The report will contain method descriptions, spectra and identified peptides from the trypsin digests, and the HPLC chromatograms for quantitation. Yes they are related I posted the results on this board here; http: The blue tops from the same company sometimes good sometimes not.
It would need an analysis for every batch. Thats why they decided not to mess any more with all these "brands". Also Hygetropin is made by 3 different guys. This analysis is done in the German lab I also used for BoS and it has cost our middleman euro to exactly know what he sells. Thanks for your reply.. This is all quiet confusing, I'm a member of another board and the message on there has always been that the brown tops.
I've also recently seen a blood serum test for the brown tops which was Well on the blog you can find some analyses from Dr Lins. He is a good friend. And you can also find the history of Hygetropin on our blog. Are you refering to the Growth hormone part 1, 2 and 3 articals? Just to confirm and sorry for going over whats probably old ground..
Dr lins are the. The ones in the attached are these fakes? The Hygenes in the pic have a batch number of and a expiry.. These are being punted on another board, but i have my reservations to be honest. Sorry for opening this topic again. But as you said before, RonnyT, the sequence matters of this amino acid chain. I totally understand this but I don't see the point where you get the informations out of this?
But purity means having these proteins in it, but not in the sequence GH must be, right? Can you provide me a conclusion out of your analysis about this sequence and chinese GH stuff? They analyse on different things, first they do the identifcation, that means they run a crs a high quality standard that is very very pure. They messure the molecular weight of that standard aa and if the molecular weight MW of the sample is the same they match and you can be sure that it is somatroipne.
This is called "identification" graphs the Standard and the Blue Top. Then they look for the area of the peak and if the sample has more peaks then one related protein Then you can calculate the dosis. I have made no pics from those out of the PDF report.