Thin-Layer Chromatography of Ecdysteroids: Detection and IdentificationQuantification of ecdysteroids and retinoic acids at picograms per individual is typically achieved with radioimmunoassay methods. However, those methods cannot identify individual types of ecdysteroids or provide an absolute concentration, which poses problems for comparative assays such as the metabolic profiling approach for toxicity testing. The method described in the present paper, based on liquid chromatography-electrospray ionization-triple quadrupole mass spectrometry, was developed to allow the quantification in whole daphnids extracts of ecdysteroids hydroxyecdysone, winstrol gains, ponasterone A and retinoic acid sum of isomers. The described liquid chromatography-triple quadrupole mass spectrometry method achieved quantification ecdysteroids chromatography ranging from to winstrol gains ecdysteroids chromatography -1 for ecdysteroids chromatography and 5 ng mL -1 for retinoic acids in spiked matrix blanks. Derivatization; Ecdysteroids; Retinoic acid; Solid-core columns; Tandem mass spectrometry; Ultra high performance liquid ecdysteroids chromatography. National Center for Biotechnology InformationU. Didn't get the message?
Capillary gas chromatography-mass spectrometry of ecdysteroids - ScienceDirect
In particular, improvements in scanning densitometers and radioactivity detectors have resulted in a versatile, rapid and quantitative technique which complements other chromatographic methods such as gas and high performance liquid chromatography.
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